Protocol

Watch on Youtube

1, Antibody beads handling for immunodepletion (Movie)

This method enables you to asve your precious antibody and also to avoid cell extract dilution.

 

 


 

2, Protein Phosphatase assay protocol (PDF)

This describes the standard PPase assay in our lab.
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3, Plasmid vector for bacterial expression of 8xhis-tagged protein

  (Go "contact" for request)

You can clone a gene of interest and  check its protein expression with this plasmid. Multiple cloning site is similar to pGEX-6P1, so very easy to move a ORF between them.

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